4TYU
Homodimeric Single Domain Antibody (sdAb) against Staphylococcal enterotoxin B (SEB)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR-H |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2012-01-12 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.54 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 67.300, 72.240, 116.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.330 - 2.130 |
R-factor | 0.2402 |
Rwork | 0.239 |
R-free | 0.26316 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dka |
RMSD bond length | 0.006 |
RMSD bond angle | 1.035 |
Data scaling software | PROTEUM PLUS |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.130 | 2.220 |
High resolution limit [Å] | 2.120 | 2.120 |
Rmerge | 0.139 | 0.365 |
Number of reflections | 15098 | |
<I/σ(I)> | 12.74 | 4.88 |
Completeness [%] | 92.6 | 60.9 |
Redundancy | 6.45 | 2.49 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 290 | 0.1 M citric acid pH 4.1, 1.6 M ammonium sulfate, 20 % glycerol |