4TXW

Crystal structure of CBM32-4 from the Clostridium perfringens NagH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	2005-09-12
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	35.102, 64.709, 37.723
Unit cell angles	90.00, 94.52, 90.00

Refinement procedure

Resolution	19.617 - 1.400
R-factor	0.15
Rwork	0.149
R-free	0.17060
Structure solution method	SIRAS
RMSD bond length	0.006
RMSD bond angle	1.073
Phasing software	SHARP
Refinement software	PHENIX (1.9_1678)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.620	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.052	0.263
Number of reflections	31314
<I/σ(I)>	14.2	2.4
Completeness [%]	94.6	75.2
Redundancy	3.58	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	12% PEG 20K 0.1M MES pH 6.5