4TXQ

Crystal structure of LIP5 N-terminal domain complexed with CHMP1B MIM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	193.15
Detector technology	CCD
Collection date	2012-12-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	52.936, 55.536, 195.359
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.076 - 2.210
R-factor	0.1955
Rwork	0.193
R-free	0.22660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4txp
RMSD bond length	0.009
RMSD bond angle	1.224
Refinement software	PHENIX ((phenix.refine: dev_1593))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.076	2.280
High resolution limit [Å]	2.208	2.208
Rmerge	0.110	0.550
Number of reflections	29702
<I/σ(I)>	19.4	3.2
Completeness [%]	99.9	100
Redundancy	7.3	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	277.15	Protein mixture was added in 1:1 ratio with a solution of 16% MPD, 0.1 M Tris and equilibrated against a mother liquid of 8% MPD, 0.1 M Tris