4TXQ
Crystal structure of LIP5 N-terminal domain complexed with CHMP1B MIM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 193.15 |
Detector technology | CCD |
Collection date | 2012-12-14 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.936, 55.536, 195.359 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.076 - 2.210 |
R-factor | 0.1955 |
Rwork | 0.193 |
R-free | 0.22660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4txp |
RMSD bond length | 0.009 |
RMSD bond angle | 1.224 |
Refinement software | PHENIX ((phenix.refine: dev_1593)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.076 | 2.280 |
High resolution limit [Å] | 2.208 | 2.208 |
Rmerge | 0.110 | 0.550 |
Number of reflections | 29702 | |
<I/σ(I)> | 19.4 | 3.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.3 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277.15 | Protein mixture was added in 1:1 ratio with a solution of 16% MPD, 0.1 M Tris and equilibrated against a mother liquid of 8% MPD, 0.1 M Tris |