4TXP

Crystal structure of LIP5 N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	193.15
Detector technology	CCD
Collection date	2011-12-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97919
Spacegroup name	P 65 2 2
Unit cell lengths	76.381, 76.381, 355.938
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.460 - 3.010
R-factor	0.2502
Rwork	0.248
R-free	0.28640
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.060
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.460	3.250
High resolution limit [Å]	3.010	3.010
Rmerge	0.100	0.600
Number of reflections	13124
<I/σ(I)>	18.8	3.6
Completeness [%]	98.8	99.8
Redundancy	6.9	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277.15	7.5-9% PEG 8000, 20% 1,4 butanediol, 0.1M cacodylate, 0.2M sodium acetate