4TXK
Construct of MICAL-1 containing the monooxygenase and calponin homology domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2013-04-14 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.233, 50.174, 96.966 |
Unit cell angles | 90.00, 101.16, 90.00 |
Refinement procedure
Resolution | 26.806 - 2.878 |
R-factor | 0.206 |
Rwork | 0.203 |
R-free | 0.27210 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.289 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 26.820 | 50.000 | 2.870 |
High resolution limit [Å] | 2.820 | 7.650 | 2.820 |
Rmerge | 0.184 | 0.057 | 0.640 |
Total number of observations | 52018 | ||
Number of reflections | 14602 | ||
<I/σ(I)> | 4.2 | ||
Completeness [%] | 96.2 | 97.2 | 65.1 |
Redundancy | 3.6 | 3.5 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 2000 MME, HEPES buffer |