4TWZ

Crystal Structure Analysis of E Coli. RecA Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-11-29
Detector	MAR CCD 165 mm
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	103.719, 103.719, 72.061
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.810 - 2.800
R-factor	0.168
Rwork	0.168
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.700
Refinement software	CNS (1.3)

Data quality characteristics

	Overall
Low resolution limit [Å]	99.000
High resolution limit [Å]	2.800
Number of reflections	11005
<I/σ(I)>	25.5
Completeness [%]	99.5
Redundancy	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	24% PEG 400, 10% GLYCEROL, 10MM MAGNESIUM CHLORIDE, 0.1M MES, PH 6.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K