4TWL
Crystal structure of dioscorin complexed with ascorbate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2011-02-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 83.505, 157.103, 83.485 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.110 |
R-factor | 0.20476 |
Rwork | 0.201 |
R-free | 0.26604 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.941 |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.110 |
Number of reflections | 31531 |
<I/σ(I)> | 51.6 |
Completeness [%] | 99.0 |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 278 | BICINE, PEG400, ammonium sulfate |