4TWJ
The structure of Sir2Af2 bound to a myristoylated histone peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-27 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.033 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.478, 77.251, 45.904 |
Unit cell angles | 90.00, 110.74, 90.00 |
Refinement procedure
Resolution | 42.930 - 1.650 |
R-factor | 0.18091 |
Rwork | 0.180 |
R-free | 0.20547 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ma3 |
RMSD bond length | 0.004 |
RMSD bond angle | 1.012 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.930 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Number of reflections | 30123 | |
<I/σ(I)> | 13.24 | 4.15 |
Completeness [%] | 99.7 | 97.89 |
Redundancy | 3.3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | 0.1 M Na-acetate pH 4.8, 14-18% (v/v) 2-propanol, and 14-15% (w/v) PEG 6,000 |