4TWI
The structure of Sir2Af1 bound to a succinylated histone peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-10 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.610, 51.362, 57.687 |
| Unit cell angles | 90.00, 101.85, 90.00 |
Refinement procedure
| Resolution | 21.940 - 1.790 |
| R-factor | 0.19374 |
| Rwork | 0.192 |
| R-free | 0.22113 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ici |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.002 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.940 | 1.849 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Number of reflections | 23269 | |
| <I/σ(I)> | 7.36 | 1.85 |
| Completeness [%] | 95.4 | 83.73 |
| Redundancy | 2.3 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris pH 8.5, 200 mM MgCl2, and 20% (w/v) PEG 8,000 |
