4TWB
Sulfolobus solfataricus ribose-phosphate pyrophosphokinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-2 |
Synchrotron site | MAX II |
Beamline | I911-2 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2010-01-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.03908 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.217, 92.846, 138.338 |
Unit cell angles | 90.00, 90.91, 90.00 |
Refinement procedure
Resolution | 19.984 - 2.802 |
R-factor | 0.2361 |
Rwork | 0.235 |
R-free | 0.26470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u9y |
RMSD bond length | 0.009 |
RMSD bond angle | 1.306 |
Data scaling software | SCALEPACK |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.089 | 0.590 |
Number of reflections | 48432 | |
<I/σ(I)> | 14 | 2.4 |
Completeness [%] | 97.9 | 98.1 |
Redundancy | 4.1 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5 | 293 | Protein: 12.7 mg/mL 50 mM Tris/HCl pH 7.6 10 mM AMP 10 mM ribose 5-phosphate Buffer: 0.2 M diammonium citrate pH 5.0 Precipitant: 14 % PEG10000 |