4TWB
Sulfolobus solfataricus ribose-phosphate pyrophosphokinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2010-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.03908 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.217, 92.846, 138.338 |
| Unit cell angles | 90.00, 90.91, 90.00 |
Refinement procedure
| Resolution | 19.984 - 2.802 |
| R-factor | 0.2361 |
| Rwork | 0.235 |
| R-free | 0.26470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u9y |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.306 |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.089 | 0.590 |
| Number of reflections | 48432 | |
| <I/σ(I)> | 14 | 2.4 |
| Completeness [%] | 97.9 | 98.1 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 293 | Protein: 12.7 mg/mL 50 mM Tris/HCl pH 7.6 10 mM AMP 10 mM ribose 5-phosphate Buffer: 0.2 M diammonium citrate pH 5.0 Precipitant: 14 % PEG10000 |
