4TVZ
Crystal Structure of SCARB2 in Neural Condition (pH7.5)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-08 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.960, 63.723, 219.925 |
| Unit cell angles | 90.00, 99.75, 90.00 |
Refinement procedure
| Resolution | 34.883 - 3.006 |
| R-factor | 0.2616 |
| Rwork | 0.259 |
| R-free | 0.30440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f7b |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.604 |
| Data reduction software | SCALEPACK |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.131 | 0.072 | 0.637 |
| Number of reflections | 24297 | ||
| <I/σ(I)> | 5.6 | ||
| Completeness [%] | 92.7 | 98.2 | 93.8 |
| Redundancy | 3.4 | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1 mol/L HEPES, 25%(w/v) PEG 3350 |
