4TVV
Crystal structure of LppA from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-17 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.650, 55.490, 131.420 |
| Unit cell angles | 90.00, 99.61, 90.00 |
Refinement procedure
| Resolution | 48.259 - 1.400 |
| R-factor | 0.1435 |
| Rwork | 0.143 |
| R-free | 0.16860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f41 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.132 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 264769 | |
| <I/σ(I)> | 15.9 | 2.3 |
| Completeness [%] | 96.0 | 90.5 |
| Redundancy | 4.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 40 mM KH2PO4 ph 4.1, 16% PEG 8000, 20% glycerol |
