4TVQ
CCM3 in complex with CCM2 LD-like motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.855, 113.552, 120.034 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.490 - 2.800 |
R-factor | 0.241 |
Rwork | 0.239 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3l8i |
RMSD bond length | 0.002 |
RMSD bond angle | 0.583 |
Data reduction software | HKL-2000 (2000.0.98.705a) |
Data scaling software | HKL-2000 (2000.0.98.705a) |
Phasing software | PHASER (ccp4-6.4.0) |
Refinement software | PHENIX ((PHENIX.REFINE: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.107 | 0.937 |
Number of reflections | 130452 | |
<I/σ(I)> | 16 | 1.654 |
Completeness [%] | 99.0 | 97.5 |
Redundancy | 6.2 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 15-20% PEG3350, 0.1-0.2M POTASSIUM FLUORIDE, PH 7.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |