4TVF
OxyB from Actinoplanes teichomyceticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-30 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9718 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 153.830, 45.300, 60.430 |
| Unit cell angles | 90.00, 102.46, 90.00 |
Refinement procedure
| Resolution | 43.360 - 1.900 |
| R-factor | 0.22972 |
| Rwork | 0.229 |
| R-free | 0.25071 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lfk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.874 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.360 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.480 | |
| Number of reflections | 32255 | |
| <I/σ(I)> | 13.24 | 3.27 |
| Completeness [%] | 99.6 | 98.8 |
| Redundancy | 3.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 90 mM Mes pH 6.0, 13.5% PEG 3,350 18% glycerol |
