4TVC
N-terminally truncated dextransucrase DSR-E from Leuconostoc mesenteroides NRRL B-1299 in complex with gluco-oligosaccharides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.310, 100.140, 186.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.200 - 1.850 |
| R-factor | 0.16334 |
| Rwork | 0.161 |
| R-free | 0.20455 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ttq |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.840 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.380 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 109405 | |
| <I/σ(I)> | 8.7 | 2 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 285 | 15% PEG 3350, 0.1M ammonium nitrate |
