4TV3
Isolated p110a subunit of PI3Ka provides a platform for structure-based drug design
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 95.15 |
| Detector technology | PIXEL |
| Collection date | 2013-08-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.038, 136.773, 142.644 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.410 - 2.850 |
| R-factor | 0.21 |
| Rwork | 0.208 |
| R-free | 0.25000 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 142.640 | 3.010 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Number of reflections | 27194 | |
| <I/σ(I)> | 25.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | PEG 6K, 0.6 M Na Formate, 0.1M CHES, pH 9.0-10 and 5 mM TCEP, pH 7.0 at a temperature of 20 degC. |
