4TUX

drosophila stem-loop binding protein complexed with histone mRNA stem-loop

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 43 21 2
Unit cell lengths	74.951, 74.951, 173.479
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.538 - 3.080
R-factor	0.2612
Rwork	0.255
R-free	0.31720
RMSD bond length	0.016
RMSD bond angle	1.380
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.150
High resolution limit [Å]	3.080	3.080
Rmerge	0.099	0.500
Number of reflections	9127
<I/σ(I)>	33.7
Completeness [%]	93.4	60.4
Redundancy	12.3	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	16% (wt/vol) PEG 1000, 0.2 M Ca(Ac)2, 50 mM cacodylic acid, pH 6.5