4TUW
drosophila stem-loop binding protein complexed with histone mRNA stem-loop, phospho mimic of TPNK and C-terminal region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 74.810, 74.810, 161.285 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.733 - 2.902 |
| R-factor | 0.2194 |
| Rwork | 0.214 |
| R-free | 0.26310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.168 |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.470 | |
| Number of reflections | 10448 | |
| <I/σ(I)> | 13.6 | 3 |
| Completeness [%] | 97.6 | 81.6 |
| Redundancy | 7.2 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 16% (wt/vol) PEG3350, 0.2 M Ca(Ac)2, 50 mM cacodylate acid, pH 6.5 |
