4TUO
Crystal structure of monoclonal antibody against neuroblastoma associated antigen.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2013-12-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.88563 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.087, 61.089, 65.358 |
| Unit cell angles | 107.79, 113.43, 95.68 |
Refinement procedure
| Resolution | 56.280 - 1.550 |
| R-factor | 0.16826 |
| Rwork | 0.166 |
| R-free | 0.20637 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f8t |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.061 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.280 | 1.605 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.068 | 0.433 |
| Number of reflections | 99734 | |
| <I/σ(I)> | 11 | 2.7 |
| Completeness [%] | 90.5 | 89.7 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Bis-tris, PEG 3350, NaCl |
