4TUK

Crystal structure of monoclonal antibody against neuroblastoma associated antigen.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	80
Detector technology	PIXEL
Collection date	2013-12-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	41.583, 42.539, 59.523
Unit cell angles	86.01, 73.92, 87.39

Refinement procedure

Resolution	57.090 - 1.600
R-factor	0.16441
Rwork	0.162
R-free	0.20598
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f8t
RMSD bond length	0.021
RMSD bond angle	2.110
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.090	1.657
High resolution limit [Å]	1.600	1.600
Rmerge	0.056	0.260
Number of reflections	46752
<I/σ(I)>	17.38	3.76
Completeness [%]	90.7	90.66
Redundancy	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Hepes, PEG 4000, isopropanol