4TUJ
Crystal structure of monoclonal antibody against neuroblastoma associated antigen.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 80 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-06-17 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.380, 69.820, 78.000 |
| Unit cell angles | 111.45, 101.14, 90.35 |
Refinement procedure
| Resolution | 24.777 - 1.890 |
| R-factor | 0.1816 |
| Rwork | 0.179 |
| R-free | 0.23240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f8t |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.388 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.840 | 1.990 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.069 | 0.548 |
| Number of reflections | 60908 | |
| <I/σ(I)> | 9.66 | 1.85 |
| Completeness [%] | 90.9 | 84.54 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Bis-Tris containing PEG 5000 |
