4TUE
Crystal structure of ASL-SufJ bound to Codon ACC-U on the Ribosome
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-22 |
| Detector | RAYONIX MX300HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 211.282, 448.357, 616.604 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.802 - 3.500 |
| R-factor | 0.2156 |
| Rwork | 0.214 |
| R-free | 0.24990 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.923 |
| Data scaling software | XSCALE |
| Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.600 | |
| High resolution limit [Å] | 3.500 | 35.000 | 3.500 |
| Rmerge | 0.231 | 0.103 | 1.147 |
| Rmeas | 0.254 | 0.122 | 1.285 |
| Total number of observations | 4247078 | ||
| Number of reflections | 732004 | 445 | 58239 |
| <I/σ(I)> | 5.69 | 3.69 | 1.19 |
| Completeness [%] | 99.7 | 51.3 | 98.6 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl |
