4TUD

Crystal structure of ASL-SufJ bound to Codon ACC-C on the Ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-22
Detector	RAYONIX MX300HS
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	210.464, 450.700, 621.065
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.847 - 3.600
R-factor	0.2066
Rwork	0.205
R-free	0.24410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3I8F 3I8G 3I8H 3I8I
RMSD bond length	0.006
RMSD bond angle	0.983
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		3.700
High resolution limit [Å]	3.600	35.000	3.600
Rmerge	0.187	0.056	0.908
Rmeas	0.208	0.070	1.011
Total number of observations	3587145
Number of reflections	675413	441	52893
<I/σ(I)>	7.84	4.96	1.93
Completeness [%]	99.9	50.9	99.8
Redundancy	5.3		5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl