4TTX
N-terminal domain of C. Reinhardtii SAS-6 homolog bld12p variant G94C K146C (NN19)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.590, 95.550, 91.430 |
| Unit cell angles | 90.00, 112.61, 90.00 |
Refinement procedure
| Resolution | 63.257 - 2.498 |
| R-factor | 0.2182 |
| Rwork | 0.216 |
| R-free | 0.26640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q0y |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.480 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.260 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 2.017 | |
| Number of reflections | 48246 | |
| <I/σ(I)> | 6.78 | 0.72 |
| Completeness [%] | 99.6 | 97.7 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | PEG6000, MES-NaOH |
