4TTX

N-terminal domain of C. Reinhardtii SAS-6 homolog bld12p variant G94C K146C (NN19)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	87.590, 95.550, 91.430
Unit cell angles	90.00, 112.61, 90.00

Refinement procedure

Resolution	63.257 - 2.498
R-factor	0.2182
Rwork	0.216
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3q0y
RMSD bond length	0.001
RMSD bond angle	0.480
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.260	2.560
High resolution limit [Å]	2.500	2.500
Rmerge		2.017
Number of reflections	48246
<I/σ(I)>	6.78	0.72
Completeness [%]	99.6	97.7
Redundancy	3.8	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	PEG6000, MES-NaOH