4TTW
N-terminal domain of C. Reinhardtii SAS-6 homolog bld12p K105C F145C (NN18)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-10-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9999 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 98.040, 29.300, 63.720 |
Unit cell angles | 90.00, 126.99, 90.00 |
Refinement procedure
Resolution | 20.784 - 1.200 |
R-factor | 0.1641 |
Rwork | 0.163 |
R-free | 0.18860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3q0y |
RMSD bond length | 0.010 |
RMSD bond angle | 1.340 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.898 | 1.230 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 1.514 | |
Number of reflections | 45556 | |
<I/σ(I)> | 16.2 | 1.35 |
Completeness [%] | 99.0 | 95.5 |
Redundancy | 6.4 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6 | 293 | PEG3350, MES-NaOH |