4TSZ
Crystal structure of DNA polymerase sliding clamp from Pseudomonas aeruginosa with ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 79.982, 85.947, 272.202 |
| Unit cell angles | 90.01, 89.94, 116.56 |
Refinement procedure
| Resolution | 29.280 - 2.000 |
| R-factor | 0.2029 |
| Rwork | 0.202 |
| R-free | 0.22190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.110 |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.300 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 787947 | |
| <I/σ(I)> | 6.12 | 0.75 |
| Completeness [%] | 87.9 | 75.3 |
| Redundancy | 1.6 | 1.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1M sodium acetate pH 4.5 and PEG 3350 |
