4TSZ
Crystal structure of DNA polymerase sliding clamp from Pseudomonas aeruginosa with ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-20 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 79.982, 85.947, 272.202 |
Unit cell angles | 90.01, 89.94, 116.56 |
Refinement procedure
Resolution | 29.280 - 2.000 |
R-factor | 0.2029 |
Rwork | 0.202 |
R-free | 0.22190 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.110 |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.300 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 787947 | |
<I/σ(I)> | 6.12 | 0.75 |
Completeness [%] | 87.9 | 75.3 |
Redundancy | 1.6 | 1.55 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.1M sodium acetate pH 4.5 and PEG 3350 |