4TSP
Crystal structure of FraC with DHPC bound (crystal form II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-24 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 129.550, 129.550, 49.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.930 - 2.150 |
| R-factor | 0.20238 |
| Rwork | 0.200 |
| R-free | 0.24744 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vwi |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.980 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.930 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.125 | 0.570 |
| Number of reflections | 22701 | |
| <I/σ(I)> | 6.5 | 2 |
| Completeness [%] | 97.6 | 79.7 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 50 mM DHPC, 200 mM Li2SO4, 20% PEG 1000, 100 mM phosphate-citrate |
