4TSN
Crystal structure of FraC with POC bound (crystal form II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-10 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.130, 65.640, 86.700 |
| Unit cell angles | 90.00, 101.06, 90.00 |
Refinement procedure
| Resolution | 35.700 - 1.570 |
| R-factor | 0.16116 |
| Rwork | 0.160 |
| R-free | 0.19740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vwi |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.868 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.700 | 1.650 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.090 | 0.480 |
| Number of reflections | 101761 | |
| <I/σ(I)> | 9.6 | 2.3 |
| Completeness [%] | 98.4 | 82.2 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM POC, 3 mM DDM, 200 mM Li2SO4, 24% PEG 8000, 100 sodium acetate |
