4TSL
Crystal structure of FraC with POC bound (crystal form I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 64.740, 64.740, 219.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.370 - 1.600 |
| R-factor | 0.14954 |
| Rwork | 0.149 |
| R-free | 0.17238 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vwi |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.336 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.370 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.103 | 0.800 |
| Number of reflections | 62950 | |
| <I/σ(I)> | 17.5 | 3.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.8 | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM POC, 3 mM DDM, 200 mM Li2SO4, 100 mM ammonium formate, 24% PEG 8000, 100 sodium acetate |
