4TSE

Crystal Structure of the Mib Repeat Domain of Mind bomb 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.988
Spacegroup name	P 21 21 2
Unit cell lengths	51.320, 86.420, 86.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.150 - 2.057
R-factor	0.1918
Rwork	0.190
R-free	0.22930
RMSD bond length	0.012
RMSD bond angle	1.311
Data scaling software	SCALEPACK
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.090
High resolution limit [Å]	2.050	5.560	2.050
Rmerge	0.113	0.044	0.644
Total number of observations	149146
Number of reflections	24570
<I/σ(I)>	6.2		2.6
Completeness [%]	99.7	97.2	99.8
Redundancy	6.1	5.8	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	293	24% v/v PEK2K MME, 0.8M HCOONa using 6 mg/ml protein