4TSC
Structure of a lysozyme antibody complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 78.629, 129.734, 59.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.870 - 1.920 |
R-factor | 0.21 |
Rwork | 0.209 |
R-free | 0.23190 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.110 |
Refinement software | BUSTER (2.11.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.240 | 2.150 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.306 | |
Number of reflections | 46461 | |
<I/σ(I)> | 10.4 | 3.5 |
Completeness [%] | 99.2 | 98.9 |
Redundancy | 4.5 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100mM PCTP pH 8.5, 25% w/v Peg3350 Magnesium Chloride 200mM |