4TSC
Structure of a lysozyme antibody complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 78.629, 129.734, 59.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.870 - 1.920 |
| R-factor | 0.21 |
| Rwork | 0.209 |
| R-free | 0.23190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.110 |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.240 | 2.150 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.306 | |
| Number of reflections | 46461 | |
| <I/σ(I)> | 10.4 | 3.5 |
| Completeness [%] | 99.2 | 98.9 |
| Redundancy | 4.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100mM PCTP pH 8.5, 25% w/v Peg3350 Magnesium Chloride 200mM |
