4TSB
Structure of a lysozyme antibody complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-11 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 80.600, 99.910, 75.090 |
Unit cell angles | 90.00, 110.12, 90.00 |
Refinement procedure
Resolution | 34.890 - 1.950 |
R-factor | 0.1954 |
Rwork | 0.194 |
R-free | 0.22670 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.100 |
Refinement software | BUSTER (2.11.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.890 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.391 | |
Number of reflections | 34734 | |
<I/σ(I)> | 12.2 | 2.2 |
Completeness [%] | 85.4 | 39.1 |
Redundancy | 4.1 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100mM PCPT pH 5.5, 200mM MgCl2, 22 % w/v PEG 3350 |