4TS6
Crystal structure of the RIM C2A domain from Drosophila.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-09-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.999 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.080, 38.700, 131.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 65.680 - 1.920 |
R-factor | 0.186 |
Rwork | 0.184 |
R-free | 0.21820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bwq |
RMSD bond length | 0.007 |
RMSD bond angle | 1.190 |
Data reduction software | XDS |
Data scaling software | Aimless (3.11) |
Phasing software | MrBUMP |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 65.680 | 1.970 | |
High resolution limit [Å] | 1.920 | 8.590 | 1.920 |
Rmerge | 0.057 | 0.025 | 0.643 |
Rmeas | 0.066 | 0.029 | 0.751 |
Total number of observations | 90891 | ||
Number of reflections | 13188 | 269 | 1756 |
<I/σ(I)> | 12.39 | 28.68 | 1.97 |
Completeness [%] | 99.6 | 95.7 | 99.6 |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M tri-lithium citrate, 20% w/v PEG 3350 |