4TS4
Crystal structure of the hydrolase domain of 10-formyltetrahydrofolate dehydrogenase (wild-type) from zebrafish
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL12B2 |
Synchrotron site | SPring-8 |
Beamline | BL12B2 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2010-02-10 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 104.665, 53.878, 60.632 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.025 - 1.750 |
R-factor | 0.1813 |
Rwork | 0.180 |
R-free | 0.20070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s3i |
RMSD bond length | 0.007 |
RMSD bond angle | 1.302 |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.440 | |
Number of reflections | 35347 | |
<I/σ(I)> | 33.8 | 4 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.9 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | Bis-Tris (0.1~0.2 M, pH 5.5) and PEG3350 (25~29%, w/v) |