4TRK

Structure of C. elegans HIM-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-30
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 61
Unit cell lengths	73.140, 73.140, 110.450
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.570 - 1.751
R-factor	0.1596
Rwork	0.158
R-free	0.18530
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.224
Data reduction software	XDS
Data scaling software	SCALA
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.750
Number of reflections	33729
<I/σ(I)>	13.2
Completeness [%]	100.0
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.8 M Na malonate