4TRH
The Legionella effector SidC defines a unique family of ubiquitin ligases important for bacterial phagosomal remodeling
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-20 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.987 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.230, 132.685, 171.369 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.900 - 2.030 |
R-factor | 0.21499 |
Rwork | 0.213 |
R-free | 0.24929 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.765 |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 35.900 |
High resolution limit [Å] | 2.030 |
Number of reflections | 90100 |
<I/σ(I)> | 25 |
Completeness [%] | 95.2 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.1 M cacodylate pH 5.6, 7.5% PEG6000, and 10 mM DTT |