4TRG
the SNL domain of SidC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-20 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.533, 133.438, 170.249 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.000 - 2.590 |
R-factor | 0.19083 |
Rwork | 0.189 |
R-free | 0.23104 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.637 |
Data scaling software | HKL-2000 |
Phasing software | SHELX |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.000 | 2.660 |
High resolution limit [Å] | 2.590 | 2.600 |
Rmerge | 0.180 | |
Number of reflections | 44061 | |
<I/σ(I)> | 31.1 | 10.9 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 7.8 | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.1 M cacodylate pH 5.6, 7.5% PEG6000, and 10 mM DTT |