4TRD
Crystal structure of Staphylcoccal nuclease variant Delta+PHS I72K/V74K at pH 9.0 at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2014-04-28 |
Detector | APEX II CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.809, 60.204, 73.827 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.470 - 1.600 |
R-factor | 0.1713 |
Rwork | 0.170 |
R-free | 0.19470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bdc |
RMSD bond length | 0.017 |
RMSD bond angle | 1.863 |
Data reduction software | SAINT |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.620 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.024 | 0.183 |
Number of reflections | 19443 | |
<I/σ(I)> | 33.96 | 5.09 |
Completeness [%] | 100.0 | 100 |
Redundancy | 18.49 | 5.73 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 20% MPD, 25mM potassium phosphate, calcium chloride, pdTp |