4TRC

Sulfolobus solfataricus adenine phosphoribosyltransferase with adenine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	122
Detector technology	AREA DETECTOR
Collection date	2011-04-01
Detector	MARRESEARCH
Wavelength(s)	1.03911
Spacegroup name	P 61
Unit cell lengths	137.200, 137.200, 53.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.705 - 2.400
R-factor	0.2022
Rwork	0.200
R-free	0.24160
RMSD bond length	0.008
RMSD bond angle	0.982
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.400
Number of reflections	22808
<I/σ(I)>	28.26
Completeness [%]	99.6
Redundancy	13.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Protein 8.9 mg/mL 25 mM TRIS pH 7.6 0.1 mM EDTA 5 mM adenine Buffer 0.1 M phosphate-citrate pH 4.2 Precipitant 20 % PEG1000 0.2 M Li2SO4