4TRB
Sulfolobus solfataricus adenine phosphoribosyltransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 122 |
Detector technology | CCD |
Collection date | 2007-02-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.93100 |
Spacegroup name | P 61 |
Unit cell lengths | 125.628, 125.628, 69.707 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.721 - 2.400 |
R-factor | 0.2306 |
Rwork | 0.228 |
R-free | 0.28650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1NUL and 1VDM |
RMSD bond length | 0.008 |
RMSD bond angle | 0.985 |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.400 |
Number of reflections | 24914 |
<I/σ(I)> | 23.8 |
Completeness [%] | 100.0 |
Redundancy | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Protein: 6.2 mg/mL, 25 mM TRIS pH 7.6, 0.1 mM EDTA, Buffer: 0.1 M MES pH 6.5, Precipitant: 30 % Jeffamine M-600 |