4TR6
Crystal structure of DNA polymerase sliding clamp from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.00153 |
Spacegroup name | P 1 |
Unit cell lengths | 43.220, 68.330, 85.310 |
Unit cell angles | 65.77, 82.84, 86.79 |
Refinement procedure
Resolution | 21.960 - 1.500 |
R-factor | 0.1775 |
Rwork | 0.176 |
R-free | 0.20410 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.200 |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 42.800 |
High resolution limit [Å] | 1.500 |
Number of reflections | 135911 |
<I/σ(I)> | 16.36 |
Completeness [%] | 95.9 |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.1M bis tris pH 6.5 and 28% PEG MME 2000 |