4TR6
Crystal structure of DNA polymerase sliding clamp from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00153 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.220, 68.330, 85.310 |
| Unit cell angles | 65.77, 82.84, 86.79 |
Refinement procedure
| Resolution | 21.960 - 1.500 |
| R-factor | 0.1775 |
| Rwork | 0.176 |
| R-free | 0.20410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.800 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 135911 |
| <I/σ(I)> | 16.36 |
| Completeness [%] | 95.9 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1M bis tris pH 6.5 and 28% PEG MME 2000 |
