4TR5

C3larvin toxin, an ADP-ribosyltransferase from Paenibacillus larvae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-15
Detector	RAYONIX MX-300
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	55.850, 55.850, 120.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.019 - 2.300
R-factor	0.1888
Rwork	0.187
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g24
RMSD bond length	0.005
RMSD bond angle	0.874
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	41.020
High resolution limit [Å]	2.300
Number of reflections	9065
<I/σ(I)>	25.83
Completeness [%]	100.0
Redundancy	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	297.15	28% PEG 4000, 200 mM sodium acetate, and 100 mM Tris