4TR0
Crystal structure of GSSG-bound cGrx2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 107.848, 64.007, 44.462 |
Unit cell angles | 90.00, 103.21, 90.00 |
Refinement procedure
Resolution | 19.642 - 1.951 |
R-factor | 0.1525 |
Rwork | 0.150 |
R-free | 0.19530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kte |
RMSD bond length | 0.019 |
RMSD bond angle | 1.737 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.950 |
Number of reflections | 19791 |
<I/σ(I)> | 18.3 |
Completeness [%] | 90.6 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES, pH 7.5, 28% PEG 400 (w/v), and 0.2 M calcium chloride |