4TQU

Crystal structure of a bacterial ABC transporter involved in the import of the acidic polysaccharide alginate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	71.367, 134.177, 273.798
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.638 - 3.204
R-factor	0.2393
Rwork	0.237
R-free	0.28160
RMSD bond length	0.005
RMSD bond angle	1.057
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	3.200
Number of reflections	43105
<I/σ(I)>	23.9
Completeness [%]	97.6
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG3000, N-(2-acetamido)iminodiacetic acid NaCl