4TQQ

Photosynthetic Reaction Center from R. sphaeroides Analyzed at Room Temperature on an X-ray Transparent Microfluidic Chip

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	298
Detector technology	CCD
Collection date	2012-12-18
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 42 21 2
Unit cell lengths	102.484, 102.484, 239.939
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.814 - 2.499
R-factor	0.1645
Rwork	0.162
R-free	0.21340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2uww
RMSD bond length	0.010
RMSD bond angle	1.388
Data reduction software	HKL-2000
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.814	2.560
High resolution limit [Å]	2.499	2.500
Rmerge	0.165	0.628
Number of reflections	45251
<I/σ(I)>	10	3
Completeness [%]	95.8	90
Redundancy	4	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROFLUIDIC		293	Protein incorporated into LCP by diffusion, incubated for hours, crystallization induced by addition of Jeffamine M-600 and ammonium sulfate