4TQK
Structural basis of specific recognition of non-reducing terminal N-acetylglucosamine by an Agrocybe aegerita lection
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 1W2B |
Synchrotron site | BSRF |
Beamline | 1W2B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-01-01 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.560, 111.240, 134.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.280 - 2.100 |
R-factor | 0.1748 |
Rwork | 0.171 |
R-free | 0.24080 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.093 |
Data scaling software | SCALA |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 85.754 | 26.280 | 2.210 |
High resolution limit [Å] | 2.100 | 6.640 | 2.100 |
Rmerge | 0.089 | 0.410 | |
Rmeas | 0.166 | ||
Rpim | 0.085 | 0.042 | 0.320 |
Total number of observations | 126025 | 8206 | 8468 |
Number of reflections | 41489 | ||
<I/σ(I)> | 6.3 | 13.8 | 2.2 |
Completeness [%] | 88.8 | 96.1 | 70.4 |
Redundancy | 3 | 5.2 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 25% PEG 4000 (w/v), 6% Tacsimate pH 6.0 and 0.1M MES pH 6.0 |