4TQA
Crystal Structure of a GDP-bound G13D Oncogenic Mutant of Human GTPase KRas
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-12 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97924 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 66.157, 41.283, 114.464 |
| Unit cell angles | 90.00, 105.03, 90.00 |
Refinement procedure
| Resolution | 27.238 - 1.130 |
| R-factor | 0.1409 |
| Rwork | 0.140 |
| R-free | 0.16850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.476 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.150 |
| High resolution limit [Å] | 1.130 | 3.070 | 1.130 |
| Rmerge | 0.050 | 0.028 | 0.523 |
| Total number of observations | 489332 | ||
| Number of reflections | 111367 | ||
| <I/σ(I)> | 9.4 | ||
| Completeness [%] | 99.7 | 94.7 | 99.3 |
| Redundancy | 4.4 | 4.4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2M Sodium Acetate pH4.5, 0.1M Tris pH 8.5, 26% PEG 3,350 |
