4TQ5

Structure of a UbiA homolog from Archaeoglobus fulgidus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97800
Spacegroup name	P 31 1 2
Unit cell lengths	61.922, 61.922, 248.752
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.244 - 3.202
R-factor	0.2548
Rwork	0.251
R-free	0.28860
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.952
Data reduction software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.260
High resolution limit [Å]	3.200	8.670	3.200
Rmerge	0.128	0.030
Total number of observations	197115
Number of reflections	9401
<I/σ(I)>	4.9
Completeness [%]	99.9	100	98.7
Redundancy	21	19.6	15.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	298	12.5% PEG 20000, 100 mM MES