4TQ4
Structure of a UbiA homolog from Archaeoglobus fulgidus bound to DMAPP and Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-08 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97890 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.605, 218.922, 65.292 |
Unit cell angles | 90.00, 90.13, 90.00 |
Refinement procedure
Resolution | 39.083 - 2.502 |
R-factor | 0.2173 |
Rwork | 0.215 |
R-free | 0.25540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tq3 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.167 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
Rmerge | 0.116 | 0.069 | 0.672 |
Total number of observations | 173584 | ||
Number of reflections | 51825 | ||
<I/σ(I)> | 6.6 | ||
Completeness [%] | 99.2 | 98.8 | 98.6 |
Redundancy | 3.3 | 3.5 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 298 | 34% PEG 400, 100 mM Tris-HCL, 100 mM NaCl, 100 mM MgCl2, 1-oleoyl-rac-glycerol |