4TQ2
Structure of S-type Phycobiliprotein Lyase CPES from Guillardia theta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97794, 0.97856 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.830, 116.580, 58.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.574 - 1.950 |
| R-factor | 0.2111 |
| Rwork | 0.209 |
| R-free | 0.24520 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.544 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.574 | 19.574 | 2.000 |
| High resolution limit [Å] | 1.950 | 8.720 | 1.950 |
| Rmerge | 0.050 | 0.026 | 2.094 |
| Rmeas | 0.052 | 0.027 | 2.176 |
| Total number of observations | 266849 | ||
| Number of reflections | 20588 | 248 | 1496 |
| <I/σ(I)> | 24.26 | 77.82 | 1.42 |
| Completeness [%] | 99.7 | 89.9 | 99.6 |
| Redundancy | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 291 | 0.015M CoCl2, 0.085M sodium acetate pH 4.6, 0.85M 1,6-hexanediol, 7% glycerol |
